Human Melatonin Receptor 1A (MTNR1A) ELISA Kit from MyBioSource.com

Supplier Page

Supplier Page from
MyBioSource.com for
Human Melatonin Receptor 1A (MTNR1A) ELISA Kit

Get Pricing

Description

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MTNR1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MTNR1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MTNR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MTNR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MTNR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve